Homosassa is a census-designated place (CDP) in Citrus County, Florida, United States. The population was 2,578 at the 2010 census.
Homosassa is derived from a Seminole Indian name meaning either "river of fishes" or "pepper ridge".
In 1851, David Levy Yulee established a 5,000-acre (20 km2) sugar plantation on the Homosassa River, close to the current town of Homosassa. The plantation was worked by approximately 1,000 slaves, but Yulee was an absentee owner, spending most of his time in Fernandina. Among the crops raised were sugar cane, cotton, and citrus. The Yulee Groves were one of the first in Florida to grow sweet oranges budded from sour orange stock. The mill, which was steam-driven, operated from 1851 to 1864 and produced sugar, syrup, and molasses, the last of which was part of the rum-making process. After the Civil War ended in 1865, Yulee was imprisoned, the slaves were freed, and the site was abandoned. The remains of the plantation are preserved at the Yulee Sugar Mill Ruins Historic State Park.
Homosassa is home to Homosassa Springs Wildlife State Park, which is often home to large numbers of "migratory" manatees, which frequent the area due to its springs and relatively warm waters. The park a has been known to have Florida panthers, black bears, red wolves, alligators and flamingos.