Brooksville is a town in Noxubee County, Mississippi, United States. The population was 1,223 at the 2010 census.
The Sam D. Hamilton Noxubee National Wildlife Refuge is located west of the town, and the Black Prairie Wildlife Management Area is located to the north.
The town's name comes from the several brooks which flow nearby.
A post office was established in 1846, and the town was incorporated in 1860.
Brooksville was a stop on the Mobile and Ohio Railroad, built in the 1850s.
Brooksville has a large Holdemann Mennonite community.
Brooksville is located at 33°13′58″N 88°34′54″W (33.232853, -88.581600).
According to the United States Census Bureau, the town has a total area of 1.0 square mile (2.6 km2), all land.
At the census of 2000, there were 1,182 people, 439 households, and 305 families residing in the town. The population density was 1,189.1 people per square mile (461.0/km2). There were 480 housing units at an average density of 482.9 per square mile (187.2/km2). The racial makeup of the town was 19.12% White, 79.61% African American, 0.17% Asian, and 1.10% from two or more races. Hispanic or Latino of any race were 1.10% of the population.
There were 439 households, out of which 35.8% had children under the age of 18 living with them, 31.2% were married couples living together, 33.7% had a female householder with no husband present, and 30.5% were non-families. 28.2% of all households were made up of individuals, and 13.4% had someone living alone who was 65 years of age or older.