Mylo is a city in Rolette County, North Dakota, United States. The population was 20 at the 2010 census.
In June 1905, lots were sold for what is now the Village of Mylo. In November 1905, the Minneapolis, St. Paul and Sault Ste. Marie railroad, simply known as the "Soo" or "Soo Line", for the phonetic spelling of "Sault" in Sault Ste. Marie, came through Mylo. On November 18, 1905, crews working from the east and those working from the west met about one mile west of Mylo to complete this agricultural branch of the Soo Line. A Post Office was established in Mylo in 1905 and was in operation until its closing in 2011. The Mylo Post Office also provided the rural route service for the Towner County city of Perth, N.D. in the later years. Mylo was incorporated as a village in 1907.