Greensboro is a borough in Greene County, Pennsylvania, United States. The population was 260 at the 2010 census, down from 295 at the 2000 census.
Greensboro is located in southeastern Greene County at 39°47′34″N 79°54′44″W (39.792911, -79.912087). It is situated on the west bank of the Monongahela River, opposite New Geneva and the mouth of George's Creek. It is bordered by Monongahela Township in Greene County to the north and west, and by Nicholson Township in Fayette County to the south and east, across the Monongahela. The county and township border follows the center of the river. The closest road bridges over the river are the Masontown Bridge on Pennsylvania Route 21, 8 miles (13 km) north of Greensboro by road, and the Albert Gallatin Memorial Bridge on Pennsylvania Route 88 at Point Marion, 6 miles (10 km) south of Greensboro.
According to the United States Census Bureau, the borough has a total area of 0.15 square miles (0.38 km2), of which 0.11 square miles (0.28 km2) are land and 0.04 square miles (0.10 km2), or 26.06%, are water.
Like much of the region, Greensboro can trace its roots back to Native American settlement, to the Mingo tribes of the Northern Iroquois. What was to later become Greensboro was first known to the Mingo as "Delight", so named in recognition of the rich farmland that stretched along the banks of the Monongahela River. As early as 1752, settlers from the east and south began to stake their claims in the lands along the Monongahela.