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Harbine is a village in Jefferson County, Nebraska, United States. The population was 49 at the 2010 census.
Harbine was established in 1886 when the Chicago, Rock Island, and Pacific Railroad was extended to that point. It was named for its founder, Thomas Harbine.
Harbine is located at 40°11′30″N 96°58′26″W (40.191529, -96.973772).
According to the United States Census Bureau, the village has a total area of 0.11 square miles (0.28 km2), all land.
As of the census of 2010, there were 49 people, 21 households, and 16 families residing in the village. The population density was 445.5 inhabitants per square mile (172.0/km2). There were 25 housing units at an average density of 227.3 per square mile (87.8/km2). The racial makeup of the village was 100.0% White. Hispanic or Latino of any race were 12.2% of the population.
There were 21 households, of which 28.6% had children under the age of 18 living with them, 66.7% were married couples living together, 4.8% had a female householder with no husband present, 4.8% had a male householder with no wife present, and 23.8% were non-families. 19.0% of all households were made up of individuals, and 9.6% had someone living alone who was 65 years of age or older.