Melbourne is a home rule-class city in Campbell County, Kentucky, United States, along the south bank of the Ohio River. The population was 401 at the 2010 census. St. Anne Convent is located in Melbourne; scenes from the movie Rain Man were filmed there.
Melbourne is located in northeastern Campbell County at 39°1′54″N 84°22′7″W (39.031571, −84.368600). It is bordered by the city of Silver Grove to the west. To the north, across the Ohio River, is the unincorporated community of Coldstream, Ohio.
Kentucky Route 8 passes through Melbourne, following the Ohio River 9 miles (14 km) northwest (downstream) to Dayton and 31 miles (50 km) southeast to Augusta. Downtown Cincinnati, Ohio, is 11 miles (18 km) to the northwest via Interstate 471.
According to the United States Census Bureau, Melbourne has a total area of 0.78 square miles (2.01 km2), all land.
As of the census of 2000, there were 457 people, 150 households, and 115 families residing in the city.